Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey
Abstract Background Herpes Simplex Virus (HSV) Genital Ulcer Disease (GUD) is an important public health problem, whose interaction with HIV results in mutually enhancing epidemics.Conventional methods for detecting HSV tend to be slow and insensitive.We designed a rapid PCR-based assay to quantify and type HSV in cervicovaginal lavage (CVL) fluid